ISOLASI DAN KARAKTERISASI BAKTERI AMILOLITIK Bacillus sp. DARI TANAH RHIZOSFER DESA TEGALWATON KABUPATEN SEMARANG

Henokh Christian Prasgi; Theresia Avilla Jessica Puspitasari

doi:10.33323/indigenous.v5i2.302

Henokh Christian Prasgi 082241011004 https://orcid.org/0000-0003-1058-8517
Theresia Avilla Jessica Puspitasari Faculty of Biology, Satya Wacana Christian University

DOI: https://doi.org/10.33323/indigenous.v5i2.302

Keywords: Amylolytic Bacteria, Bacillus thuringiensis, Isolation, Morphology, Biochemical and Physiology Test

Abstract

Amylolytic bacteria have the ability to hydrolyze starch into glucose as an energy source for bacterial growth, can be found in terrestrial environments including the rhizosphere soil layer. Amylase enzymes are widely useful in industry, so exploration and research is needed related to bacteria that have amyolytic abilities. This study aims to isolate and characterize amylolytic bacteria of the genus Bacillus found in the rhizosphere soil of Tegalwaton Village, Semarang Regency. Methods carried out experimentally in the Microbiology Laboratory, Faculty of Biology, Satya Wacana Christian University include isolation and character tests of morphology, biochemistry and physiology. The results of the study contained 3 bacterial isolates with codes A1, A2 and A3 identified as Bacillus genus and have similarities with Bacillus thuringiensis, including in the amylolytic rods group bacteria, Gram positive and have spores. B. thuringiensis can ferment glucose, converting hydrogen peroxide (H₂O₂) into H₂O and O₂. Instead it cannot utilize citrate as a carbon source, it cannot oxidize glucose and forms acetyl methyl carbinol. Bacillus thuringiensis can be developed into an industrial strain for amylase enzymes. Molecular tests are needed to identify the specific species.

Downloads

Download data is not yet available.

References

Adwitiya, P., Ashwini, P., Avinash, K. A., Badri, R., Kajal, D., Vomsi, P., & Srividya, S. (2009). Mutagenesis of Bacillus thuringiensis IAM 12077 for increasing poly ( - β - ) hydroxybutyrate ( PHB ) production. Turk J Biol, 33(2009), 225–230. https://doi.org/10.3906/biy-0808-10
Aryal, S. (2019). Potassium Hydroxide Test – Principle, Procedure, Uses and Interpretation. Online access. https://microbiologyinfo.com/potassium-hydroxide-test/.
Aryal, S. (2019). Catalase Test- Principle, Uses, Procedure, Result Interpretation with Precautions. Online access. https://microbiologyinfo.com/catalase-test-principle-uses-procedure-result-interpretation-with-precautions/.
Aryal, S. (2019). Motility Test – Principle, Procedure, Uses and Interpretation. Online access. https://microbiologyinfo.com/motility-test/.
Astuti, D. T., Pujiastuti, Y., Suparman, S. H. K., Damiri, N., Nugraha, S., Sembiring, E. R., & Mulawarman. (2018). Exploration of Bacillus thuringiensis Berl. from soil and screening test its toxicity on insects of Lepidoptera order. International Symposium on Food and Agro-Biodiversity, 102(012063), 1–8. https://doi.org/doi :10.1088/1755-1315/102/1/012063
Bergey, D. H., Whitman, W. B., De, V. P., Garrity, G. M., & Jones, D. (2009). Bergey's manual of systematic bacteriology: Vol. 3. New York: Springer.
Chi, Z., Chi, Z., Liu, G., Wang, F., Ju, L., & Zhang, T. (2009). Saccharomycopsis fibuligera and its applications in biotechnology. Biotechnology advances, 27(4), 423-431.
Kamar, R., Réjasse, A., Jéhanno, I., Attieh, Z., Courtin, P., Nielsen-leroux, C., Lereclus, D., & Sanchis-borja, V. (2017). DltX of Bacillus thuringiensis Is Essential for D -Alanylation of Teichoic Acids and Resistance to Antimicrobial Response in Insects. Front. Microbiol., 8(August), 1–13. https://doi.org/10.3389/fmicb.2017.01437
Kassogué, A., Maïga, K., Traoré, D., Dicko, A. H., Fané, R., Guissou, T., Faradji, F. A., Valicente, F. H., Hamadoun, A., & Babana, A. H. (2015). Isolation and characterization of Bacillus thuringiensis ( Ernst Berliner ) strains indigenous to agricultural soils of Mali. African Journal of Agricultural Research, 10(28), 2748–2755. https://doi.org/10.5897/AJAR2015.9848
Luang-In, V., Yotchaisarn, M., Saengha, W., Udomwong, P., Deeseenthum, S., & Maneewan, K. (2019). Isolation and identification of amylase-producing bacteria from soil in Nasinuan community forest, Maha Sarakham, Thailand. Biomedical and Pharmacology Journal, 12(3), 1061–1068. https://doi.org/10.13005/bpj/1735
Mac-Williams, M. P. (2009). Citrate Test Protocol. In American Society for Microbiology (Issue December 2009, pp. 1–7).
Pokhrel, B., Wanjare, P., Singh S. (2013). Isolation, screening and characterization of promising-amylase producing bacteria from sewage enriched soil. Int. J. Adv. Biotechnol. Res., 4(2): 286-290.
Serin, B., Akcan, N. and Uyar, F. (2012). Production and optimization of α-amylase from Bacillus circulans ATCC 4516 with solid state fermentation. J. Biol. Chem. 40: 393-400.
Van Der Maarel, M. J., Van der Veen, B., Uitdehaag, J. C., Leemhuis, H., & Dijkhuizen, L. (2002). Properties and applications of starch-converting enzymes of the α-amylase family. Journal of biotechnology, 94(2), 137-155.