ISOLASI DAN SKRINING ACTINOMYCETES ENDOFITIK PADA AKAR MANGROVE YANG BERPOTENSI MENGHASILKAN ENZIM HIDROLITIK
Keywords:
Actinomycetes Endofitik, Enzim Hidrolitik, Imbuhan Pakan Ternak, Perakaran Mangrove.
Abstract
Actinomycetes termasuk kelompok mikroorganisme prokariotik yang telah lama dimanfaatkan dalam berbagai bidang industri karena kemampuannya untuk menghasilkan metabolit primer dan sekunder yang sangat beragam. Di alam, kelompok mikroorganisme ini paling banyak hidup di tanah namun telah berhasil diisolasi pula dari dalam tubuh makhluk hidup sebagai organisme endofitik. Pada penelitian ini, isolasi Actinomycetes Endofitik telah berhasil dilakukan dari perakaran Mangrove yang hidup di perairan pantai Oesapa, kota Kupang. Proses isolasi menghasilkan 3 isolat Actinomycetes Endofitik, yaitu: isolat A11, A12 dan A2, yang kemudian dilanjutkan ke tahapan skrining untuk melihat kemampuan katalitiknya dalam mendegradasi substrat selulosa, amilum, lipid dan protein. Berdasarkan hasil uji skrining, ketiga isolat memiliki minimal salah satu dari enzim hidrolitik yang diujikan. Isolat A11 menunjukkan hasil uji positif terhadap enzim Amilase yang tinggi dan juga terhadap enzim Lipase. Isolat A12 menunjukkan hasil uji positif terhadap enzim Amilase yang tinggi dan juga terhadap enzim Lipase dan Protease. Isolat A2 mengandung keempat enzim hidrolitik yang diujikan sehingga dapat dianggap sebagai jenis isolat yang paling ideal apabila akan dikembangkan produktivitas enzim hidrolitiknya untuk dikembangkan sebagai imbuhan pakan ternak (feed additives). Hasil penelitian ini diharapkan dapat memberikan informasi dan juga kontribusi bagi pengembangan komoditas peternakan di daerah Nusa Tenggara Timur (NTT) sesuai dengan program yang dicanangkan oleh Kementerian Pertanian dan Pemerintah Provinsi NTT misalnya melalui pengembangan produk pakan ternak berkualitas yang memanfaatkan sumber daya lokal.
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References
Afzal, I., Shinwari, Z. K., Sikandar, S., & Shahzad, S. (2019). Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological Research, 221, 36–49. https://doi.org/10.1016/j.micres.2019.02.001
Aghamirian, M. R., & Ghiasian, S. A. (2009). Isolation and Characterization of Medically Important Aerobic Actinomycetes in Soil of Iran (2006—2007). The Open Microbiology Journal, 3, 53–57. https://doi.org/10.2174/1874285800903010053
Alnahdi, H. S. (2012). Isolation and screening of extracellular proteases produced by new Isolated Bacillus sp. Journal of Applied Pharmaceutical Science. https://doi.org/10.7324/JAPS.2012.2915
Ayuningrum, D., Sabdaningsih, A., & Jati, O. (2021). Screening of actinobacteria-producing amylolytic enzyme in sediment from Litopenaeus vannamei (Boone, 1931) ponds in Rembang District, Central Java, Indonesia. Biodiversitas Journal of Biological Diversity, 22(4), Article 4. https://doi.org/10.13057/biodiv/d220427
Basyuni, M., Oku, H., Baba, S., Takara, K., & Iwasaki, H. (2007). Isoprenoids of Okinawan mangroves as lipid input into estuarine ecosystem. Journal of Oceanography, 63(4), 601–608. https://doi.org/10.1007/s10872-007-0053-2
Berezin, V., Abdukhakimova, D., Trenozhnikova, L., Bogoyavlenskiy, A., Turmagambetova, A., Issanov, A., & Azizan, A. (2019). Antiviral activities of extremophilic actinomycetes extracts from Kazakhstan’s unique ecosystems against influenza viruses and paramyxoviruses. Virology Journal, 16(1), 150. https://doi.org/10.1186/s12985-019-1254-1
Bruckner, M. Z. (2017). Gram Staining. Microscopy. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html
Dewi, T. K., Agustiani, D., & Antonius, S. (2017). Secondary Metabolites Production by Actinomycetes and their Antifungal Activity. KnE Life Sciences, 256–264. https://doi.org/10.18502/kls.v3i4.713
Goedecke, C. (2016). Why Does Iodine Turn Starch Blue? ChemViews. https://doi.org/10.1002/chemv.201600103
Gohel, H. R., Contractor, C. N., Ghosh, S. K., & Braganza, V. J. (2014). A comparative study of various staining techniques for determination of extra cellular cellulase activity on Carboxy Methyl Cellulose (CMC) agar plates. 6.
Hastuti, U. S., Sangur, K., & Khasanah, H. N. (2015). BIODIVERSITY AND ENZYME ACTIVITY OF INDIGENOUS CELLULOLYTIC AND AMYLOLYTIC BACTERIAS IN DECAYED MANGROVE STEM WASTE PRODUCT AT WAAI SEASHORE, AMBON ISLAND. KnE Life Sciences, 433–438. https://doi.org/10.18502/kls.v2i1.188
Hidayatullah, A. R. (2019). ISOLASI ACTINOMYCETES DARI SEDIMEN MANGROVE UJUNG PANGKAH DI GRESIK [Skripsi, Universitas Airlangga]. http://lib.unair.ac.id
Jette, J. F., & Ziomek, E. (1994). Determination of lipase activity by a rhodamine-triglyceride-agarose assay. Analytical Biochemistry, 219(2), 256–260. https://doi.org/10.1006/abio.1994.1265
Joseph, J., Abirami, B., Manigundan, K., Gopikrishnan, V., & Radhakrishnan, M. (2021). Chapter 9 - Antimicrobial peptides from Actinobacteria: Current status and future prospects. In S. Das & H. R. Dash (Eds.), Microbial and Natural Macromolecules (pp. 205–231). Academic Press. https://doi.org/10.1016/B978-0-12-820084-1.00009-0
Kishore, P. (2011). Isolation, characterization and identification of Actinobacteria of Mangrove ecosystem, Bhitarkanika, Odisha [MSc]. http://ethesis.nitrkl.ac.in/2337/
Luang-In, V., Yotchaisarn, M., Saengha, W., Udomwong, P., Deeseenthum, S., & Maneewan, K. (2019). Isolation and Identification of Amylase-producing Bacteria from Soil in Nasinuan Community Forest, Maha Sarakham, Thailand. Biomedical and Pharmacology Journal, 12(3), 1061–1068.
Madhu, S., Evans, H. A., Doan-Nguyen, V. V. T., Labram, J. G., Wu, G., Chabinyc, M. L., Seshadri, R., & Wudl, F. (2016). Infinite Polyiodide Chains in the Pyrroloperylene–Iodine Complex: Insights into the Starch–Iodine and Perylene–Iodine Complexes. Angewandte Chemie International Edition, 55(28), 8032–8035. https://doi.org/10.1002/anie.201601585
Matsumoto, A., & Takahashi, Y. (2017). Endophytic actinomycetes: Promising source of novel bioactive compounds. The Journal of Antibiotics, 70(5), 514–519. https://doi.org/10.1038/ja.2017.20
Mochon, A. B., Sussland, D., & Saubolle, M. A. (2016). Aerobic Actinomycetes of Clinical Significance. Microbiology Spectrum, 4(4). https://doi.org/10.1128/microbiolspec.DMIH2-0021-2015
Mukhtar, S., Zaheer, A., Aiysha, D., Abdulla Malik, K., & Mehnaz, S. (2017). Actinomycetes: A Source of Industrially Important Enzymes. Journal of Proteomics & Bioinformatics, 10(12). https://doi.org/10.4172/jpb.1000456
Ortenberg, E., & Telsch, B. (2003). 42—Taste and odour problems in potable water. In D. Mara & N. Horan (Eds.), Handbook of Water and Wastewater Microbiology (pp. 777–793). Academic Press. https://doi.org/10.1016/B978-012470100-7/50043-1
Perwendha, R., Oetari, A., & Sjamsuridzal, W. (2020). Skimmed milk-degrading ability of Rhizopus azygosporus UICC 539 at various temperatures. 050006. https://doi.org/10.1063/5.0007872
Pramiadi, D., & Yulianti, E. (2014). Isolasi dan uji aktivitas enzim lipase termostabil dari bakteri termofilik pasca erupsi Merapi. 11.
Putri, A. L., & Setiawan, R. (2019). Isolation and screening of actinomycetes producing cellulase and xylanase from Mamasa soil, West Sulawesi. IOP Conference Series: Earth and Environmental Science, 308(1), 012035. https://doi.org/10.1088/1755-1315/308/1/012035
Ravikumar, S., Inbaneson, S., Uthiraselvam, M., Priya, S., Ramu, A., & Banerjee, M. (2011). Diversity of endophytic actinomycetes from Karangkadu mangrove ecosystem and its antibacterial potential against bacterial pathogens. Journal of Pharmacy Research, 4, 294–296.
S P, M., Sreeshan, A., & Augustine, A. (2016). Comparative Lipid Isolation Protocols from Rhizophora mucronata L. of Dharmadam Estuary and Lipid Profiling in the Context of a Non Mangrove Mangifera indica. Biochemistry & Analytical Biochemistry, 5. https://doi.org/10.4172/2161-1009.1000286
Saenger, W. (1984). The structure of the blue starch-iodine complex | SpringerLink. Naturwissenschaften, 71, 31–36.
Sauer, D. B., & Burroughs, R. (1986). Dininfection of Seed Surface with Sodium Hypochlorite. PHYTOPATHOLOGY, 76(7).
Song, L., Jiang, N., Wei, S., Lan, Z., & Pan, L. (2020). Isolation, Screening, and Identification of Actinomycetes with Antifungal and Enzyme Activity Assays against Colletotrichum dematium of Sarcandra glabra. MICROBIOLOGY, 48(1), 37–43.
Swarna, D., & Gnanadoss, J. J. (2020). Screening and Molecular Characterization of Actinomycetes from Mangrove Soil Producing Industrially Important Enzymes. Journal of Scientific Research, 64(02), 87–95. https://doi.org/10.37398/JSR.2020.640211
Tankeshwar, A. (2021). Colony Morphology of Bacteria • Microbe Online. Microbe Online. https://microbeonline.com/colony-morphology-bacteria-describe-bacterial-colonies/
Tille, P. M. (2017). Bailey & Scott’s Diagnostic Microbiology. Elsevier. https://fk.uii.ac.id/mikrobiologi/materi/sterilisasi/
Tripathi, N., & Sapra, A. (2021). Gram Staining. In StatPearls. StatPearls Publishing. http://www.ncbi.nlm.nih.gov/books/NBK562156/
Yakupova, E. I., Bobyleva, L. G., Vikhlyantsev, I. M., & Bobylev, A. G. (2019). Congo Red and amyloids: History and relationship. Bioscience Reports, 39(1), BSR20181415. https://doi.org/10.1042/BSR20181415
Zhuang, W., Yu, X., Hu, R., Luo, Z., Liu, X., Zheng, X., Xiao, F., Peng, Y., He, Q., Tian, Y., Yang, T., Wang, S., Shu, L., Yan, Q., Wang, C., & He, Z. (2020). Diversity, function and assembly of mangrove root-associated microbial communities at a continuous fine-scale. Npj Biofilms and Microbiomes, 6(1), 1–10. https://doi.org/10.1038/s41522-020-00164-6
Aghamirian, M. R., & Ghiasian, S. A. (2009). Isolation and Characterization of Medically Important Aerobic Actinomycetes in Soil of Iran (2006—2007). The Open Microbiology Journal, 3, 53–57. https://doi.org/10.2174/1874285800903010053
Alnahdi, H. S. (2012). Isolation and screening of extracellular proteases produced by new Isolated Bacillus sp. Journal of Applied Pharmaceutical Science. https://doi.org/10.7324/JAPS.2012.2915
Ayuningrum, D., Sabdaningsih, A., & Jati, O. (2021). Screening of actinobacteria-producing amylolytic enzyme in sediment from Litopenaeus vannamei (Boone, 1931) ponds in Rembang District, Central Java, Indonesia. Biodiversitas Journal of Biological Diversity, 22(4), Article 4. https://doi.org/10.13057/biodiv/d220427
Basyuni, M., Oku, H., Baba, S., Takara, K., & Iwasaki, H. (2007). Isoprenoids of Okinawan mangroves as lipid input into estuarine ecosystem. Journal of Oceanography, 63(4), 601–608. https://doi.org/10.1007/s10872-007-0053-2
Berezin, V., Abdukhakimova, D., Trenozhnikova, L., Bogoyavlenskiy, A., Turmagambetova, A., Issanov, A., & Azizan, A. (2019). Antiviral activities of extremophilic actinomycetes extracts from Kazakhstan’s unique ecosystems against influenza viruses and paramyxoviruses. Virology Journal, 16(1), 150. https://doi.org/10.1186/s12985-019-1254-1
Bruckner, M. Z. (2017). Gram Staining. Microscopy. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html
Dewi, T. K., Agustiani, D., & Antonius, S. (2017). Secondary Metabolites Production by Actinomycetes and their Antifungal Activity. KnE Life Sciences, 256–264. https://doi.org/10.18502/kls.v3i4.713
Goedecke, C. (2016). Why Does Iodine Turn Starch Blue? ChemViews. https://doi.org/10.1002/chemv.201600103
Gohel, H. R., Contractor, C. N., Ghosh, S. K., & Braganza, V. J. (2014). A comparative study of various staining techniques for determination of extra cellular cellulase activity on Carboxy Methyl Cellulose (CMC) agar plates. 6.
Hastuti, U. S., Sangur, K., & Khasanah, H. N. (2015). BIODIVERSITY AND ENZYME ACTIVITY OF INDIGENOUS CELLULOLYTIC AND AMYLOLYTIC BACTERIAS IN DECAYED MANGROVE STEM WASTE PRODUCT AT WAAI SEASHORE, AMBON ISLAND. KnE Life Sciences, 433–438. https://doi.org/10.18502/kls.v2i1.188
Hidayatullah, A. R. (2019). ISOLASI ACTINOMYCETES DARI SEDIMEN MANGROVE UJUNG PANGKAH DI GRESIK [Skripsi, Universitas Airlangga]. http://lib.unair.ac.id
Jette, J. F., & Ziomek, E. (1994). Determination of lipase activity by a rhodamine-triglyceride-agarose assay. Analytical Biochemistry, 219(2), 256–260. https://doi.org/10.1006/abio.1994.1265
Joseph, J., Abirami, B., Manigundan, K., Gopikrishnan, V., & Radhakrishnan, M. (2021). Chapter 9 - Antimicrobial peptides from Actinobacteria: Current status and future prospects. In S. Das & H. R. Dash (Eds.), Microbial and Natural Macromolecules (pp. 205–231). Academic Press. https://doi.org/10.1016/B978-0-12-820084-1.00009-0
Kishore, P. (2011). Isolation, characterization and identification of Actinobacteria of Mangrove ecosystem, Bhitarkanika, Odisha [MSc]. http://ethesis.nitrkl.ac.in/2337/
Luang-In, V., Yotchaisarn, M., Saengha, W., Udomwong, P., Deeseenthum, S., & Maneewan, K. (2019). Isolation and Identification of Amylase-producing Bacteria from Soil in Nasinuan Community Forest, Maha Sarakham, Thailand. Biomedical and Pharmacology Journal, 12(3), 1061–1068.
Madhu, S., Evans, H. A., Doan-Nguyen, V. V. T., Labram, J. G., Wu, G., Chabinyc, M. L., Seshadri, R., & Wudl, F. (2016). Infinite Polyiodide Chains in the Pyrroloperylene–Iodine Complex: Insights into the Starch–Iodine and Perylene–Iodine Complexes. Angewandte Chemie International Edition, 55(28), 8032–8035. https://doi.org/10.1002/anie.201601585
Matsumoto, A., & Takahashi, Y. (2017). Endophytic actinomycetes: Promising source of novel bioactive compounds. The Journal of Antibiotics, 70(5), 514–519. https://doi.org/10.1038/ja.2017.20
Mochon, A. B., Sussland, D., & Saubolle, M. A. (2016). Aerobic Actinomycetes of Clinical Significance. Microbiology Spectrum, 4(4). https://doi.org/10.1128/microbiolspec.DMIH2-0021-2015
Mukhtar, S., Zaheer, A., Aiysha, D., Abdulla Malik, K., & Mehnaz, S. (2017). Actinomycetes: A Source of Industrially Important Enzymes. Journal of Proteomics & Bioinformatics, 10(12). https://doi.org/10.4172/jpb.1000456
Ortenberg, E., & Telsch, B. (2003). 42—Taste and odour problems in potable water. In D. Mara & N. Horan (Eds.), Handbook of Water and Wastewater Microbiology (pp. 777–793). Academic Press. https://doi.org/10.1016/B978-012470100-7/50043-1
Perwendha, R., Oetari, A., & Sjamsuridzal, W. (2020). Skimmed milk-degrading ability of Rhizopus azygosporus UICC 539 at various temperatures. 050006. https://doi.org/10.1063/5.0007872
Pramiadi, D., & Yulianti, E. (2014). Isolasi dan uji aktivitas enzim lipase termostabil dari bakteri termofilik pasca erupsi Merapi. 11.
Putri, A. L., & Setiawan, R. (2019). Isolation and screening of actinomycetes producing cellulase and xylanase from Mamasa soil, West Sulawesi. IOP Conference Series: Earth and Environmental Science, 308(1), 012035. https://doi.org/10.1088/1755-1315/308/1/012035
Ravikumar, S., Inbaneson, S., Uthiraselvam, M., Priya, S., Ramu, A., & Banerjee, M. (2011). Diversity of endophytic actinomycetes from Karangkadu mangrove ecosystem and its antibacterial potential against bacterial pathogens. Journal of Pharmacy Research, 4, 294–296.
S P, M., Sreeshan, A., & Augustine, A. (2016). Comparative Lipid Isolation Protocols from Rhizophora mucronata L. of Dharmadam Estuary and Lipid Profiling in the Context of a Non Mangrove Mangifera indica. Biochemistry & Analytical Biochemistry, 5. https://doi.org/10.4172/2161-1009.1000286
Saenger, W. (1984). The structure of the blue starch-iodine complex | SpringerLink. Naturwissenschaften, 71, 31–36.
Sauer, D. B., & Burroughs, R. (1986). Dininfection of Seed Surface with Sodium Hypochlorite. PHYTOPATHOLOGY, 76(7).
Song, L., Jiang, N., Wei, S., Lan, Z., & Pan, L. (2020). Isolation, Screening, and Identification of Actinomycetes with Antifungal and Enzyme Activity Assays against Colletotrichum dematium of Sarcandra glabra. MICROBIOLOGY, 48(1), 37–43.
Swarna, D., & Gnanadoss, J. J. (2020). Screening and Molecular Characterization of Actinomycetes from Mangrove Soil Producing Industrially Important Enzymes. Journal of Scientific Research, 64(02), 87–95. https://doi.org/10.37398/JSR.2020.640211
Tankeshwar, A. (2021). Colony Morphology of Bacteria • Microbe Online. Microbe Online. https://microbeonline.com/colony-morphology-bacteria-describe-bacterial-colonies/
Tille, P. M. (2017). Bailey & Scott’s Diagnostic Microbiology. Elsevier. https://fk.uii.ac.id/mikrobiologi/materi/sterilisasi/
Tripathi, N., & Sapra, A. (2021). Gram Staining. In StatPearls. StatPearls Publishing. http://www.ncbi.nlm.nih.gov/books/NBK562156/
Yakupova, E. I., Bobyleva, L. G., Vikhlyantsev, I. M., & Bobylev, A. G. (2019). Congo Red and amyloids: History and relationship. Bioscience Reports, 39(1), BSR20181415. https://doi.org/10.1042/BSR20181415
Zhuang, W., Yu, X., Hu, R., Luo, Z., Liu, X., Zheng, X., Xiao, F., Peng, Y., He, Q., Tian, Y., Yang, T., Wang, S., Shu, L., Yan, Q., Wang, C., & He, Z. (2020). Diversity, function and assembly of mangrove root-associated microbial communities at a continuous fine-scale. Npj Biofilms and Microbiomes, 6(1), 1–10. https://doi.org/10.1038/s41522-020-00164-6
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Published
2022-03-04
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How to Cite
Vertygo, S. (2022). ISOLASI DAN SKRINING ACTINOMYCETES ENDOFITIK PADA AKAR MANGROVE YANG BERPOTENSI MENGHASILKAN ENZIM HIDROLITIK. INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI, 4(3), 79-91. https://doi.org/10.33323/indigenous.v4i3.264
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